anti pmtor  (Bioss)

Journal: iScience

Article Title: Synaptotagmin-7 deficit causes insulin hypoactivity and contributes to behavioral alterations in mice

doi: 10.1016/j.isci.2025.112354

Figure Lengend Snippet: Signaling pathways involved in the genesis of mood cycling (A) Schematic showing comparative analysis of hippocampal gene expression profiles between manic and depressive Syt7 KO mice. GeneM, comparison between the WT mice and the manic Syt7 KO mice. GeneD, comparison between the WT mice and the depressive Syt7 KO mice. (B and C) Heatmap (B) and distribution (C) of genes differentially expressed between GeneM and GeneD. (D) Expression of representative BD-related genes in manic and depressive Syt7 KO mice. (E) Representative significantly enriched KEGG pathways showing bi-directional alterations in the manic and depressive episodes in Syt7 KO mice. (F and G) Schematic (F) and heatmap (G) showing the comparative analysis of transcripts bi-directionally regulated in drug-induced/Syt7 deficiency-induced manic and depressive mice. GeneR, comparison between the WT mice with and without the Ro25 treatment in the dark phase. GeneB, comparison between the WT mice with and without the BMS-536924 treatment in the light phase. (H) Representative significantly enriched KEGG pathways showing bi-directional alterations between the drug-induced/Syt7-deficiency-induced manic and depressive mice. (I) Mapping of representative KEGG pathways for network analysis. (J) Bi-directional regulation of representative genes within featured KEGG pathways in mice with drug-induced/Syt7-deficiency-induced mania or depression. (K and L) Immunoblots (left) and quantitative analysis (right) of mammalian targets of rapamycin (mTOR) (K) and eukaryotic elongation factor 2 (eEF2) (L) phosphorylation in the hippocampus of WT and Syt7 KO mice. n = 3. Student’s t test; ∗ p < 0.05; ∗∗ p < 0.001; error bars, SEM.

Article Snippet: Primary antibodies were as follows: mouse monoclonal anti-Actin antibody (1:5000, Abcam, #ab6276), rabbit polyclonal anti-mTOR antibody (1:500, Cell Signaling, #2972), rabbit polyclonal anti- p -mTOR antibody (1:500, Cell Signaling, #2971), rabbit polyclonal anti-eEF2 antibody (1:1000, Cell Signaling, #2332S), rabbit polyclonal anti- p -eEF2 antibody (1:1000, Cell Signaling, #2331S).

Techniques: Protein-Protein interactions, Gene Expression, Comparison, Expressing, Western Blot, Phospho-proteomics

Journal: iScience

Article Title: Synaptotagmin-7 deficit causes insulin hypoactivity and contributes to behavioral alterations in mice

doi: 10.1016/j.isci.2025.112354

Figure Lengend Snippet: Signaling pathways involved in the genesis of mood cycling (A) Schematic showing comparative analysis of hippocampal gene expression profiles between manic and depressive Syt7 KO mice. GeneM, comparison between the WT mice and the manic Syt7 KO mice. GeneD, comparison between the WT mice and the depressive Syt7 KO mice. (B and C) Heatmap (B) and distribution (C) of genes differentially expressed between GeneM and GeneD. (D) Expression of representative BD-related genes in manic and depressive Syt7 KO mice. (E) Representative significantly enriched KEGG pathways showing bi-directional alterations in the manic and depressive episodes in Syt7 KO mice. (F and G) Schematic (F) and heatmap (G) showing the comparative analysis of transcripts bi-directionally regulated in drug-induced/Syt7 deficiency-induced manic and depressive mice. GeneR, comparison between the WT mice with and without the Ro25 treatment in the dark phase. GeneB, comparison between the WT mice with and without the BMS-536924 treatment in the light phase. (H) Representative significantly enriched KEGG pathways showing bi-directional alterations between the drug-induced/Syt7-deficiency-induced manic and depressive mice. (I) Mapping of representative KEGG pathways for network analysis. (J) Bi-directional regulation of representative genes within featured KEGG pathways in mice with drug-induced/Syt7-deficiency-induced mania or depression. (K and L) Immunoblots (left) and quantitative analysis (right) of mammalian targets of rapamycin (mTOR) (K) and eukaryotic elongation factor 2 (eEF2) (L) phosphorylation in the hippocampus of WT and Syt7 KO mice. n = 3. Student’s t test; ∗ p < 0.05; ∗∗ p < 0.001; error bars, SEM.

Article Snippet: Rabbit polyclonal anti- p -mTOR , Cell Signaling , Cat# 2971; RRID: AB_330970.

Techniques: Protein-Protein interactions, Gene Expression, Comparison, Expressing, Western Blot, Phospho-proteomics

Journal: Molecular Cancer

Article Title: The anti-diabetic PPARγ agonist Pioglitazone inhibits cell proliferation and induces metabolic reprogramming in prostate cancer

doi: 10.1186/s12943-025-02320-y

Figure Lengend Snippet: PPARγ agonist Pioglitazone reprograms primary and metastatic PCa cell metabolism and induces an epithelial phenotype in 22RV1 cells. a Workflow scheme for metabolic assessment based on the Seahorse assay, NRM untargeted metabolomics, and radiotracer cell uptake of 22RV1 and PC3 cells upon PPAR agonist Bezafibrate, Tesaglitazar, and Pioglitazone treatment (24 hours, 100 µM, vehicle control = 0.2 % DMSO). b Metabolic profile of 22RV1 and PC3 cells comparing basal oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) calculated from fourth basal timepoint before Oligomycin injection. c, d, e Mitochondrial stress-induced OCR ( c ), ATP production ( d ) and ECAR in 22RV1 cells under control culture conditions and PPAR agonist treatment ( e ). Oligomycin was used as an inhibitor of ATP synthase/complex V, FCCP for maximal respiration by chemical uncoupling of the mitochondrial membrane gradient, and Rotenone Antimycin A (R/A) for non-mitochondrial respiration by complex I and III inhibition. f, g, h Mitochondrial stress-induced OCR responses ( f ), ATP production ( g ), and ECAR in PC3 cells ( h ) as described for 22RV1 cells. i, j, k Glycolysis test in 22RV1 after glucose and pyruvate starvation and treatment of PPAR agonists, as described above. Glucose injection after starvation serves as a measure for glycolysis, Oligomycin as the remaining contribution of OXPHOS to cellular energy production, and 2-deoxy-glucose (2-DG) for non-glycolytic ECAR contribution. ECAR profiles upon glucose injection following 75 minutes of glucose and pyruvate starvation ( i ), normalized glycolysis of 22RV1 cells treated with PPAR agonists ( j ), and normalized OCR ( k) . l, m, n Glycolysis tests in PC3 cells as described for 22RV1 cells ( i, j, k) . o Score plot of partial least squares-discriminant analysis (PLS-DA) model for bucketed NMR spectral data from 22RV1 (left; the model parameters for the two components fitted were as follows: R2Y = 0.498, Q2Y = 0.301) and PC3 cell extracts (right; the model parameters for the three components fitted were as follows: R2Y = 0.696, Q2Y = 0.499) treated with PPAR agonists as described above. p Relative abundance of short-chain fatty acids, resulting from the untargeted metabolomics of PPAR agonist treated 22RV1 (left) and PC3 cells. q [ 18 F]FTHA uptake after 24 hours of treatment with each PPAR agonist in 22RV1 and PC3 cells. r Western Blot analysis of mTOR, phospho mTOR (Ser2448), AMPKα, phospho AMPKα (Thr172), E-Cadherin, and Vimentin expression in control and PPAR agonist treated 22RV1 and PC3 cells, β-Actin was used as a loading control. One representative experiment of the Seahorse assay profile is shown. Significance was evaluated by one-way ANOVA (ns = not significant p > 0.05, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001). Data are representative of the means ± SD biological triplicates

Article Snippet: Rabbit polyclonal phospho mTOR (Ser2448) , Cell Signaling , #2971.

Techniques: Control, Injection, Membrane, Inhibition, Western Blot, Expressing

Journal: Cell reports

Article Title: Restraint of inflammasome-driven cytokine responses through the mRNA stability protein TTP

doi: 10.1016/j.celrep.2025.115340

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-phospho-mTOR (Ser2448) , Cell Signaling Technology , Cat# 2971; RRID:AB_330970.

Techniques: Recombinant, CyQUANT Assay, LDH Cytotoxicity Assay, Enzyme-linked Immunosorbent Assay, Software

Journal: International Journal of Molecular Sciences

Article Title: The Effect of Valine on the Synthesis of α-Casein in MAC-T Cells and the Expression and Phosphorylation of Genes Related to the mTOR Signaling Pathway

doi: 10.3390/ijms26073179

Figure Lengend Snippet: Detailed information of antibodies.

Article Snippet: , P-mTOR , bioss , bs-3494R , 5% egg white , 1:500.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: The Effect of Valine on the Synthesis of α-Casein in MAC-T Cells and the Expression and Phosphorylation of Genes Related to the mTOR Signaling Pathway

doi: 10.3390/ijms26073179

Figure Lengend Snippet: Effects of varying valine concentrations on the expression levels of Rag small G Protein genes upstream in the mTOR signaling pathway. ( A – D ) represent the relative mRNA expression levels of four protein genes related to Rag small G proteins upstream of the mTOR signaling pathway: RRAGA , RRAGB , RRAGC and RRAGD . The error bars represent the SD ( n = 3). ANOVA was used to detect the differences between groups, and the LSD method was also used for uncorrected exploratory pairwise comparisons, while the Duncan method was used for conservative multiple comparison corrections. Different lowercase letters indicate significant differences ( p < 0.05), while different uppercase letters indicate significant differences ( p < 0.01).

Article Snippet: , P-mTOR , bioss , bs-3494R , 5% egg white , 1:500.

Techniques: Expressing, Comparison

Journal: International Journal of Molecular Sciences

Article Title: The Effect of Valine on the Synthesis of α-Casein in MAC-T Cells and the Expression and Phosphorylation of Genes Related to the mTOR Signaling Pathway

doi: 10.3390/ijms26073179

Figure Lengend Snippet: Effects of varying valine concentrations on the relative expression levels of mTORC1 complex-related genes. The error bars represent the SD ( n = 3). ( A – C ) represent the relative mRNA expression levels of the mTORC1 complex-related protein genes, mTOR , MLST8 , and RPTOR , respectively. ANOVA was used to detect the differences between groups, and the LSD method was combined for uncorrected exploratory pairwise comparisons, while the Duncan method was used for conservative multiple comparison corrections. Different uppercase letters indicate significant differences ( p < 0.01).

Article Snippet: , P-mTOR , bioss , bs-3494R , 5% egg white , 1:500.

Techniques: Expressing, Comparison

Journal: International Journal of Molecular Sciences

Article Title: The Effect of Valine on the Synthesis of α-Casein in MAC-T Cells and the Expression and Phosphorylation of Genes Related to the mTOR Signaling Pathway

doi: 10.3390/ijms26073179

Figure Lengend Snippet: Effects of varying valine concentrations on the relative expression levels of downstream genes in the mTOR signaling pathway. ( A – E ) represent the relative mRNA expression levels of the five key downstream targets of the mTOR pathway: EIF4EBP1 , EIF4E , S6K1 , EEF2 , and RPTOR . The error bars represent the SD ( n = 3). ANOVA was used to detect the differences between groups, and the LSD method was combined for uncorrected exploratory pairwise comparisons, while the Duncan method was used for conservative multiple comparison corrections. Different lowercase letters indicate significant differences ( p < 0.05), while different uppercase letters indicate significant differences ( p < 0.01).

Article Snippet: , P-mTOR , bioss , bs-3494R , 5% egg white , 1:500.

Techniques: Expressing, Comparison

Journal: International Journal of Molecular Sciences

Article Title: The Effect of Valine on the Synthesis of α-Casein in MAC-T Cells and the Expression and Phosphorylation of Genes Related to the mTOR Signaling Pathway

doi: 10.3390/ijms26073179

Figure Lengend Snippet: Effects of different valine concentrations on the phosphorylation of downstream proteins in the mTOR signaling pathway. ( A ) Western blotting was used to detect the protein expression levels of phosphorylated mTOR (P-mTOR) and phosphorylated S6K1 (P-S6K1). ( B ) The phosphorylation level of mTOR protein (P-mTOR/mTOR) was quantified and calculated using β-actin and mTOR as internal references. ( C ) The phosphorylation level of S6K1 protein (P-S6K1/S6K1) was quantified and calculated using β-actin and S6K1 as internal references. ( D ) Western blotting was used to detect the protein expression levels of phosphorylated 4EBP1 (P-4EBP1) and phosphorylated RPS6 (P-RPS6). ( E ) The phosphorylation level of 4EBP1 protein (P-4EBP1/4EBP1) was quantified and calculated using β-actin and 4EBP1 as internal references. ( F ) The phosphorylation level of RPS6 protein (P-RPS6/RPS6) was quantified and calculated using β-actin and RPS6 as internal references. ( G ) Western blotting was used to detect the protein expression levels of phosphorylated eEF2 (P-eEF2). ( H ) The phosphorylation level of eEF2 protein (P-eEF2/eEF2) was quantified and calculated using β-actin and eEF2 as internal references. The error bars represent the SD ( n = 3). ANOVA was used to detect the differences between groups, and the LSD method was combined for uncorrected exploratory pairwise comparisons, while the Duncan method was used for conservative multiple comparison corrections. Different lowercase letters indicate significant differences ( p < 0.05), while different uppercase letters indicate significant differences ( p < 0.01).

Article Snippet: , P-mTOR , bioss , bs-3494R , 5% egg white , 1:500.

Techniques: Western Blot, Expressing, Comparison

Journal: International Journal of Molecular Sciences

Article Title: The Effect of Valine on the Synthesis of α-Casein in MAC-T Cells and the Expression and Phosphorylation of Genes Related to the mTOR Signaling Pathway

doi: 10.3390/ijms26073179

Figure Lengend Snippet: Effects of valine and rapamycin on the phosphorylation levels of mTOR downstream proteins. ( A ) The phosphorylation levels of mTOR (P-mTOR) in MAC-T cells treated with varying concentrations of rapamycin were assessed using Western blotting. ( B ) The P-mTOR level (P-mTOR/mTOR) in MAC-T cells treated with 100 nM rapamycin was quantified and calculated using β-actin and mTOR as internal references. ( C ) Western blotting was used to detect the protein expression levels of P-mTOR and P-S6K1. ( D ) The P-mTOR level (P-mTOR/mTOR) was quantified and calculated using β-actin and mTOR as internal references. ( E ) The P-S6K1 level (P-S6K1/S6K1) was quantified and calculated using β-actin and S6K1 as internal references. ( F ) Western blotting was used to detect the protein expression levels of P-4EBP1 and P-RPS6. ( G ) The P-4EBP1 level (P-4EBP1/4EBP1) was quantified and calculated using β-actin and 4EBP1 as internal references. ( H ) The P-RPS6 level (P-RPS6/RPS6) was quantified and calculated using β-actin and RPS6 as internal references. ( I ) Western blotting was used to detect the protein expression levels of P-eEF2 and α-casein. ( J ) The P-eEF2 level (P-eEF2/eEF2) was quantified and calculated using β-actin and eEF2 as internal references. ( K ) The α-casein protein expression level was quantified using β-actin as an internal reference. The error bars represent the SD ( n = 3). ANOVA was used to detect the differences between groups, and the LSD method was combined for uncorrected exploratory pairwise comparisons, while the Duncan method was used for conservative multiple comparison corrections. Different lowercase letters indicate significant differences ( p < 0.05), while different uppercase letters indicate significant differences ( p < 0.01).

Article Snippet: , P-mTOR , bioss , bs-3494R , 5% egg white , 1:500.

Techniques: Western Blot, Expressing, Comparison

Journal: International Journal of Molecular Sciences

Article Title: The Effect of Valine on the Synthesis of α-Casein in MAC-T Cells and the Expression and Phosphorylation of Genes Related to the mTOR Signaling Pathway

doi: 10.3390/ijms26073179

Figure Lengend Snippet: Primer sequences of internal reference genes and target genes.

Article Snippet: , P-mTOR , bioss , bs-3494R , 5% egg white , 1:500.

Techniques: Sequencing

Journal: International Journal of Molecular Sciences

Article Title: The Effect of Valine on the Synthesis of α-Casein in MAC-T Cells and the Expression and Phosphorylation of Genes Related to the mTOR Signaling Pathway

doi: 10.3390/ijms26073179

Figure Lengend Snippet: Detailed information of antibodies.

Article Snippet: , P-mTOR , bioss , bs-3494R , 5% egg white , 1:500.

Techniques: